A simple, rapid and stability indicating RP-UPLC method was developed for the Simultaneous quantitative determination of chlorthalidone, Amlodipine besylate and Telmisartan HCl from their innovative combined tablet Formulation. The separation was acheived by using 100 mm x 2.1 mm, 1.7 µm RP-Sheild C-18 column with a simple gradient method at 235 nm wavelength. The mobile phase A containes a mixture of ammonium formate buffer 40 mM (PH 5.5±0.05 adjusted with formic acid ) : Acotonitrile in the ratio of 80:20 and Mobile phase B containes a mixture of ammonium formate buffer 40 mM (PH 5.5±0.05 adjusted with formic acid) : acotonitrile in the ratio of 20:80. The flow rate is 0.35 mL/min and the column temperature is maintained at 40°C. The gradient prograrmme time % of B -0/10, 0.5/10, 1.0/30, 2.8/30, 3.0/10, 3.2/10. Injection volume 1.0 µl. The total runtime for the separation of the three active compounds and their degradation products is 3.2 minutes. The method was statistically validated for precision, Linearity, Ruggedness, Robustness & Specificity. Method was showed a linear response in the concentration range of 50-150 % of three components with respect to test concentration. Quantitative and recovery studies of dosage form were also carried out and analysed, the % RSD from recovery studies was found to be less than 1. Due to simple, rapid and accuracy of the method, will be useful for routine quality control analysis.
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